M Toronto Myositis Centreat St. Michael's Hospital
← Tests & procedures Patient guide

Muscle biopsy.

A muscle biopsy takes a small sample of muscle to look at under the microscope. It is often the clearest way to confirm which type of myositis you have.

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The area is frozen first, so you should not feel sharp pain.

You stay awake. Plan for about 30 to 45 minutes, plus a short rest afterward.

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What it is

A small sample of muscle.

A muscle biopsy is a minor procedure to remove a small piece of muscle, usually from the thigh, upper arm, or shoulder. The sample is sent to the laboratory and examined under a microscope by specialists. Your team chooses a muscle that is affected by the disease but still working, often guided by your examination, your EMG, or an MRI scan.

There are two ways it may be done. A needle biopsy takes a small sample through a tiny nick in the skin. An open biopsy uses a small cut to take a slightly larger sample, which is then closed with a few stitches or skin strips.

What it tells us

Why the test is done.

Looking at the muscle directly lets the team see inflammation, muscle-fibre damage, and specific patterns that point to a particular type of myositis, such as inclusion body myositis. It can confirm the diagnosis when blood tests and other tests are not enough, and it helps rule out other muscle diseases, so your treatment is based on the right answer.

What to expect

During and after.

During the procedure
  • You lie down and the skin over the muscle is cleaned.
  • Local anaesthetic freezes the area. It stings briefly, then numbs.
  • You may feel pressure, tugging, or a deep ache while the sample is taken, but not sharp pain.
  • Taking the sample itself is quick.
Just afterward
  • A dressing is put on, with stitches or skin strips if you had an open biopsy.
  • You rest for a short time before going home.
  • The area is often sore for a few days as the freezing wears off.
Getting ready

How to prepare.

Afterward

Caring for the site.

Aftercare
  • Keep the dressing clean and dry, and follow the instructions about when to remove it or shower.
  • Rest the muscle and avoid heavy use or hard exercise for a few days.
  • Simple pain relief such as acetaminophen usually settles the soreness. Ask your team before using anti-inflammatories.
Risks
  • Common effects are soreness, bruising, and a small scar after an open biopsy.
  • Less common risks include bleeding, infection, and a patch of numbness near the site.
  • Serious problems are rare.
After the biopsy, call the clinic if:

Contact us if you notice:

Increasing pain or swelling
Growing pain, swelling, redness, or warmth around the wound.
Signs of infection
Fever, chills, or fluid or pus leaking from the site.
Bleeding that will not stop
Bleeding that soaks through the dressing and does not stop with gentle pressure.
Wound or numbness problems
The wound opening up, or numbness that spreads or does not improve.

Your results

Muscle samples take time to process, and some tests are sent to specialised laboratories, so results can take from several days to a few weeks. Your team will explain what the biopsy shows and what it means for your treatment.

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This guide is for learning, not medical advice. Your team will explain your own test, and every person’s situation is a little different. Always follow the instructions you are given.

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